The FlexStation® 3 Multi-Mode Microplate Reader integrates sensitive optics, fluid transfer and temperature control making it ideal for kinetic, cell-based fluorometric assays, such as the measurement of intracellular calcium, membrane potential and intracellular pH. Here is Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader Unit2 Predecessor to:. Variations in intracellular calcium ([Ca21] i) levels in cells were measured by calcium fluorescent dyes using the Cal-520 PBX Cal-cium Assay Kit (AAT Bioquest, Sunnyvale, CA) in a FlexStation 3 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA). Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. The reader can transfer assay reagents from. For instance, liquid transfer for endpoint and slowAll plates were removed from the incubator and allowed to cool to room temperature 10 minutes prior to being read on either the FlexStation ® 3 Multi-Mode Microplate Reader or FLIPR ® Tetra System. Multi-mode microplate readers with luminescence detection. A single-mode plate reader is a good starting point. Up to eight (96-well format) or sixteen (384-well format) wells can be monitored simultaneously before, during, and after compound addition. The FlexStation ® 3 Multi-Mode Microplate Reader offers added flexibility over dispenser-based systems by directly transferring reagents from a source plate to the read plate, which reduces reagent and consumable consumption. It has an integrated fluid transfer. . Categories: • Microplate Readers Manufacturer: Molecular Devices. Precise optics, fluidic transfer, and assay flexibility on one integrated microplate readerDownload PDF. to View Figures. The multi-mode detection modules. Multi-detection platforms often provide assay flexibility, however, throughput is often compromised— especially for applications that require integrated fluid transfer, such as calcium mobilization or other fast applications. Presented in Figures 1A and 1B are examples of how to perform a FRET assay using a Flexstation 3 in 237 which H188 is used to determine the dose-response relationship for stimulatory effects of glucagon 238 on. 1321N1 cells were used as an ‘assay-ready’ reagent. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. $373/mo. The reaction was stopped by addition of 200 µL of DNS reagent. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. After transfection for about 24 h, patch-clamp, the FlexStation 3 multi-mode microplate reader assay and calcium-imaging assay were performed. Walpole, MA, USA. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY. Materials. The FlexStation® 3 Benchtop Multi-Mode Microplate Reader from Molecular Devices combines SpectraMax® M5e Multi-Mode Microplate Reader performance with an int. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. It has an integrated fluid transfer module equipped with a multi-channel pipetter and the machine reads one column at a time. To address this concern, Molecular Devices offers the FlexStation® 3 Multi-Mode Benchtop Reader. i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita. Learn how Molecular Devices FlexStation 3 Multi-Mode Microplate Reader provides life science industry solutions. Methods Platelet preparation Collect 50 mL of blood from a healthy, consenting donor (in accordance with local ethics) into a syringe containing 4% sodium citrate (0. The ability to define individual reagents and concentrations to be delivered to each column on the cell plate allows moreThe FlexStation® 3 Benchtop Multi-Mode Microplate Reader offers added flexibility over dispenser-based systems by directly transferring reagents from a source plate to the read plate, which reduces reagent and consumable consumption. They are also fully compatible with the FlexStation 3 Multi-Mode Microplate Reader. Fluorescent membrane potential assays were performed using the FlexStation 3 multimode benchtop microplate reader and the BLUE formulation kit (catalog no. Multispan, Inc. Seller: Zantek Scientific. Multi-detection platforms often provide assay flexibility, however, throughput is often compromised— especially for applications that require integrated fluid transfer, such as calcium mobilization or other fast applications. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. She praises the equipment as an all-in-one solution for various laboratory needs, from simple microscopy to ELISA readings and even fluorescence microscopy. FlexStation 3, a next-generation multi-mode microplate reader, with liquid transfer capabilities provides a robust platform for increased throughput of this important flash luminescence assay. See our app note for details:. FlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. $18,995 USD. Multimode Reader Comparison Chart. 00. R8042, Molecular Devices). Backed by the Molecular Devices warranty, these tips are manufactured specifically to work with the FLIPR instrument under strict controls to guarantee optimal performance and quality. 00 USDTeam 3: Physiology of the Gonadotrope Axis (PAG) Team 4: Basic and Translational Myology; Team 5: Central Control of Feeding Behaviour and Energy Expenditure (C2OFEE) Team 6: Molecular and Cellular Responses to Xenobiotics (RMCX) Team 7: Degenerative Processes, Stress and Aging; Team 8: Computational Modeling of Protein-Ligand. October 2023. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. All cDNA clones. 100 fmol 384-well (75 µL) 0. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. A total of 35–40 million reads were collected for further analysis. In this application we demonstrate how PDE enzyme dilution and inhibition curves are performed with IMAP Technology using the SpectraMax ® i3, SpectraMax ® Paradigm ®, SpectraMax ® M5, and FlexStation ® 3 Multi-Mode Microplate Readers with SoftMax ® Pro Software. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 50 racks/case for 384-well (16 channel pipettor head. Ensure your assay quality with quality tips Certified RNase-/DNase. 0112-0127C 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 9 ComputerIntegration 11 InstrumentCovers 11 FluidicsModule 12 DetectionModule 16for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. 0 Mag3 < 1. In Surplus to a Global Pharmaceutical & Biotechno. Programmable liquid handling Automated pipetting ensures consistent performance over multiple assay conditions on a microplate. . Published Dec 16, 2020 | Updated Dec 16, 2020. It is ideal for medium- to high-throughput screens in academic settings. Firefly luciferase signal is an order of magnitude higher than that of Renilla. 6. Intended Use Statement The Cytation 5 is a hybrid multi-mode microplate reader. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. SpectraMax® M Series Upgradeable platform with tunable absorbance,Bath Filler and Heat Transfer Fluids; Bath Specific Platforms and Clamps; Bath Specific Tube Racks; Calibration Baths; Circulating Baths; Coliform BathsHere is a Molecular Device s FlexStation 3 Multi-Mode Microplate Reader New costs around $125,000 from VWR Predecessor to: Current model Condition: This Molecular Device s FlexStation 3 Multi-Mode Microplate R. The calibration curve for the CatchPoint cAMP Assay Kit was generated on a SpectraMax i3 Multi-Mode Microplate. The FlexStation 3 reader combines Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. 0112-0127C 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 9 ComputerIntegration 11 InstrumentCovers 11 FluidicsModule 12 DetectionModule 16FlexStation 3 Multi-Mode Microplate Reader . Cytation 3 Cell Imaging Multi-Mode Reader: Synergy 2: Synergy H1: Synergy HTX: BMG LabTech: CLARIOstar: FLUOstar OMEGA: PHERAstar FS: PHERAstar Plus: POLARstar OMEGA: Molecular Devices: FilterMax F3: FilterMax F5: FlexStation. HERM LB 500 with NaI detector Radio HPLC Detector. 425 or. SpectraMax M5. Here is a Molecular Devices FlexStation 3 Multi-Mode Microplate Reader New costs around $125,000 from VWR Predecessor to: Current model Condition: This Molecular. ZERO BIAS - scores, article reviews, protocol conditions and more. Open the inside top panel of the fluidics module. Please see the exact video and photos below. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to. This can increase throughput and efficiency, thus saving time and space in the lab. FilterMax F3 & F5 Microplate Readers. Cytation 5 combines automated microscopy and conventional microplate detection in a configurable, upgradable platform. 1 μM or 10 μM also increased intracellular levels of calcium in TRPV1-transfected HEK293 cells, which was completely reversed by a selective TRPV1 antagonist BCTC at 10 μM (Figure 2B). The ability to define individual reagents and concentrations to be delivered to each column onFlexStation_manualbook Page 1 Tuesday June 25 2013 10:22 AM FlexStation® 3 Benchtop Multi-Mode Microplate Reader User Guide 0112-0127 B June 2013…10. book Page 1 Tuesday, July 6, 2010 2:08 PM3. FlexStation 3, a next-generation multi-mode microplate reader, with liquid transfer capabilities provides a robust platform for increased throughput of this important flash luminescence assay. 0200-6263. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield and phase contrast for maximum application reach. It. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader w/ One Year Warranty (Svc Contract Available) ATG for AKTA Explorer, Purifier and FPLC PMs, Repairs and Service Contracts GE AKTA™ Crossflow System, Complete with Full One-Year Warranty (Service Contract Available) $37,500. FlexStation 3 Multi-Mode Microplate Reader | Molecular Devices Request Quote Home Resources Videos & Webinars FlexStation 3 Multi-Mode Microplate Reader Learn. $4,999. Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The SpectraMax M5, M5e, and. Readers. reporter with an internal control. It has an integrated fluid transfer module equipped with a multi-channel pipetter and the machine reads one column at a time. Users can choose from 96-well pipetting, 384-well pipetting, or both. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. 012 fmol 0. The FlexStation 3 Benchtop Multi-Mode Microplate Reader can measure absorbance, fluorescence intensity, fluorescence polarization, luminescence and time-resolved fluorescence. Flexstation 3 Multi-Mode Microplate Reader. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an. The FlexStation 3 Microplate Reader high-efficiency monochromator optics are tunable for absorbance, fluorescence intensity, fluorescence polarisation, and time-resolved fluorescence assays. It allows the user to choose between monochromator. Products : FlexStation 3 reader, SoftMax Pro Software, FlexStation (Obsolete), FlexStation II (Obsolete) This page contains the table of contents of resources available for the FlexStation Reader and Analyst Instrument Suite of Products. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. MS-3 Laser Bar Code Reader: Thermo Fisher Scientific: VisionMate 96 (Vial Rack Bottom Label 2D Reader). We demonstrate that miniaturizing the assay in 96-well half area microplates, and using the flexible liquid transfer features of the FlexStation ® 3 Multi-Mode Microplate Reader allows rapid and reliable measurement of EC 50 values. Microplate Plate Reader. The signal of DPA‐terbium was monitored by FlexStation 3 Multi‐Mode Microplate Reader with excitation/emission at 270 and 545 nm, and cutoff at 520 nm. Multimode Reader Comparison Chart. For instance, liquid transfer for endpoint and slowThe use of the FlexStation ® 3 multi-mode microplate reader has been shown to compare favorably to other methods in obtaining quantitative binding affinities between the ß-lactamase and inhibitor protein, TEM-1 and BLIP. FlexStation3Multi-ModeMicroplateReaderUserGuide. Please Inquire. FlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer. Cell Based Calcium Assay For Medium To High Throughput Screening Trp. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. Was the failure only of the Detection Estimate (Mag3) Test for the M3/M4/M5/M5e/Flex3? The M3/M4/M5/M5e/Flex3 multi-mode readers have a higher instrument noise level. Learn how Molecular Devices FlexStation 3 Multi-Mode Microplate Reader provides life science industry solutions. Listing SKU: scp-179480-f0f7cf9f-76e0-47bb-b87d-0f1cf442e6de. These lab workhorses are used in drug discovery, research, bioassay. Search Results for Flexstation 3 Fluorescent Microplate Reader on Bioz, providing objective ratings for all products used in life science research. . The FlexStation 3 system provides a high-throughput platform for studying multiple mutants and inhibitors in real timeFlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer. 99 to $1999. They provide absorbance, fluorescence, glow luminescence, and other read modes for. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Citations: Dual monochromators increase performance and assay flexibility. University of Geneva uses the FlexStation 3 and SpectraMax. Page 118 FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide 3. FlexStation® Microplate Reader and Analyst® Instrument Suite of Products Knowledge Base. Click to Contact Seller. 2. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. To address this concern, Molecular Devices offers the FlexStation® 3 multi-mode benchtop reader. FlexStation 3 0200-2420 CuvetteAbsorbance ValidationSet SpectraMaxPlus384,ABS Plus,M2,M2e,M3,M4,M5,M5e 0200-7200 Multi-ModeValidation Plate FilterMaxF3,FilterMaxF5,SpectraMaxParadigm,iD5*,i3*,DETAILED INFO AND PHOTOS FOR THIS & SIMILAR ITEMS MAY BE FOUND ATCALL US AT 617-366-2699WIDE SELECTION OF PRODUCTS. 99. , San Jose, CA, USA) 36. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Cytation™ imaging multi-mode readers combine automated digital microscopy with conventional multi-mode microplate detection, providing both phenotypic cellular information and well-based quantitative data. Preeti Bharaj This is an excellent all in one piece of table top fit equipment. com. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. has developed a large inventory of cell lines expressing specific. Lonza Luminometers Instrument MycoAlert™ Assay MycoAlert™ PLUS Assay Other Bioassays* Notes Lonza Lucetta™ Luminometer + + Cuvette Tailor -made MycoAlert™ Mode Luminometers from Other Suppliersby FLIPR or FlexStation systems using thallium-sensitive dye indicators Tl + sensitive dye Potassium ion channel: FLIPR Potassium Assay Kit: Ordering information Item: Size Part #. com. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The Molecular Devices' FlexStation 3 is a benchtop multi-mode microplate reader capable of automated fluorescence measurement in multi-well plates. It is ideal for medium- to high-throughput screens in academic settings. This auction is live!. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Eight different automated tests are provided: • Absorbance accuracy (linearity) • Precision (reproducibility) • Stray light. laboratories. The FlexStation 3 Reader FlexStation® 3 Multi-Mode Microplate Reader. Download PDF versions of our FlexStation Manuals. Warranty: NoMolecular Devices FlexStation 3 Multi-Mode Microplate Reader Unit2 - AV. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumFlexstation 3, supplied by Danaher Inc, used in various techniques. Products : FlexStation (Obsolete), FlexStation II (Obsolete), FlexStation 3 reader. FlexStation 3 Multi-Mode Microplate Reader. com. FlexStation 3, a next-generation multi-mode microplate reader, with liquid transfer capabilities provides a robust platform for increased throughput of this important flash luminescence assay. Preeti Bharaj from the University of Texas Medical Branch highly recommends the BioTek Instruments' Cytation 5 Cell Imaging Multi-Mode Reader. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Est. Asked by. In this study we have compared a variety of fluorescent calcium sensitive dyes, including the recently introduced FLIPR® Calcium 6 Assay Kit, in an endogenous CRAC channel assay in RBL cells using the FlexStation® 3 Multi-Mode Microplate Reader, a five-mode reader featuring an internal 8- or 16-channel pipettor for custom and direct transfer. It has an integrated fluid transfer module equipped with a multi-channel pipetter and the machine reads one column at a. Part 4: Applications provides detailed protocols for several FLEXstation mode experiments. Built on the proven SpectraMax® M5e optical platform, FlexStation 3 expands capacity to perform real time fast kinetic The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of the human AT1R receptor. Bioz Stars score: 98/100, based on 1 PubMed citations. GenePix 4300/4400 Microarray Scanner. Using an aspirate height of 3 mm generally yielded the best assay results, comparable to those obtained with careful manual. The multi-mode detection modules. The FlexStation 3 system provides a high-throughput platform for studying multiple mutants and inhibitors in real timeContact Us. The reducing groups were quantified at 546 nm with a FlexStation 3 Multi-Mode microplate reader. Service Vendors. item 1 Molecular Devices Analyst GT Multi-mode microplate reader Molecular Devices Analyst GT Multi-mode. With advanced dual optical systems operating above and below your microplates, the FlexStation ® 3 Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. Mg-ATP (1. Used. To address this concern, Molecular Devices offers the FlexStation® 3 Multi-Mode Benchtop Reader. A. 020 fmol 0. Flexstation 3, supplied by Danaher Inc, used in various techniques. Supplier: Molecular Devices. Bioz Stars score: 98/100, based on 1 PubMed citations. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. 1 Installation Addendum; Resources. com. 4 software and one year. Automated pipetting The FlexStation 3 reader offers automated pipetting using the 8- or 16-channel pipettor to improve assay quality and increase throughput. 5 Instrument Settings in FLEXstation Mode. It is ideal for medium- to high-throughput screens in academic settings. CHO M1WT3 cells were seeded into a 96-well black-wall, clear-bottom plate at 50,000 cells per well in 100 µL per well. FLEX3-SC - FlexStation 3 Microplate Reader with 1-Year Service Contract - FlexStation 3 Multimode Plate Reader, Molecular Devices - Each: Amazon. Standard curves obtained in a 384-well plate on FlexStation 3 in FLEX mode using Promega Dual-Luciferase Reporter Assay System. or 20 n m IVM for 15 min, rinsed with IVM-free saline, and then recorded in whole-cell current clamp mode for V-I relations using square current injection. Microplate Spectrophotometers. Two assays were developed on the SpectraMax iD3 and iD5 readers and compared to the same assays run on the industry standard FlexStation® 3 Multi-Mode Microplate Reader. Biacore 4000 high-throughput surface plasmon resonance (SPR) instrument. Preparation of Nanoparticles (NPs)Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader with One Year Warranty. Figure 2 demonstrates the flexibility of the assay as the IC 50 values did notFlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer. 60 allow live-cell real-time determinations of FRET in the kinetic mode. Multi-. Fluorescence Microplate Reader Comparison Chart. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Users have several choices when configuring the FlexStation 3 Microplate Reader. The official tips for the FLIPR system. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-reporter with an internal control. FLUOstar Omega Register or Sign In. Introduction. 0 Cross-talk D09 through D11,E09 through E11,and F09 through F11 <0. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast to address many applications and workflows. Cytation 5 combines automated microscopy and conventional microplate detection in a configurable, upgradable platform. Dr. Order Now. Click here. 96 wells, 3 colors, 4x, 12 min Laser autofocus: 96 wells, 1 color (DAPI), 4x, < 3 min 96 wells, 3 colors, 4x, < 7 min 30 s Burst mode: 10 fps, single well, single color at ≤ 5O ms integration time Image Analysis Software Option Agilent BioTek Gen5 Image+ software: image analysis Agilent BioTek Gen5 Image Prime software: advanced image analysisThe FlexStation® 3 Benchtop Multi-Mode Microplate Reader offers added flexibility over dispenser-based systems by directly transferring reagents from a source plate to the read plate, which reduces reagent and consumable consumption. ZERO BIAS - scores, article reviews, protocol conditions and moreFlexStation 3 Multi-Mode Microplate Reader: 8 -channel automated pipettor that can read mutiw ell plates in absorbance, luminescence, fluorescence and fluorescence polarization modes . October 2023. Latest Citations: For a complete list, please click here. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Plates with any of the FLIPR Calcium Assay Kits or Fluo-4 Direct Kit did not require a wash after dye loading. The reader adds up to three transfers of assay reagents from a 96- or 384. However, an upgradeable multi-mode reader which includes absorbance, fluorescence and luminescence can address all your future lab needs. FlexStation® Benchtop Multi-Mode Microplate Reader Manual Download Page. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. 2 or higher, standard edition only, no GxP). Multi-mode Microplate Readers. The High Throughput Screening Center (HTSC) is a Core facility within CDD that provides plate-based assay services, screenin resources, expertise, automation along with both siRNA and small-molecule compound libraries suitable for cell-based and biochemical assays. For instance, liquid transfer for endpoint and slowThe FlexStation® 3 Benchtop Multi-Mode Microplate Reader offers added flexibility over dispenser-based systems by directly transferring reagents from a source plate to the read plate, which reduces reagent and consumable consumption. $18,995. Page 5 If a spill occurs while a program is running, abort the program and turn off the instrument. Finally, reactions were detected with continuous monitoring of fluorescence at 25 °C for 10 min. $18,995 USD. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. FlexStation® 3 Multimode Plate Reader, Molecular Devices. Est. 2 Sensitivity. No special upgrade is required for HTRF® readout, as it is a monochromator-based instrument. The ability to define individual reagents and concentrations to be delivered to each column on the cell plate allows. You must be Signed in to. Est. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with. readings were taken using kinetic mode on the Flexstation 3 with readings being taken every. In addition, the SpectraMax iD5 can be expanded to. Calcium mobilization in WT3M1 CHO cells by carbachol, run on FlexStation 3 in 384-well microplate with the FLIPR Calcium 4 Assay Kit. Configuring the SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers for ValitaTiter Assays. FlexStation II Operator’s Manual – Rev. The Molecular Devices' FlexStation 3 is a benchtop multi-mode microplate reader capable of automated fluorescence measurement in multi-well plates. Pharmacokinetics of DHI compounds in rats plasma. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. The SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. Number of Citations*: 8350. FilterMax F3 & F5 Microplate Readers. cAMP calibration curve. 分子. The Molecular Devices' FlexStation 3 is a benchtop multi-mode microplate reader capable of automated fluorescence measurement in multi-well plates. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. Backed by the Molecular Devices warranty, these tips are manufactured specifically to work with the FlexStation 3 instrument under strict controls to guarantee optimal performance and quality. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® Europium自動マルチチャンネルピペッターと5モードリードで、多様なバイオケミカル・細胞ベースアッセイを可能にします. 0 (newest 2017 version) trial Then once you order 1 year SoftMax 7. Supplier: Molecular Devices. IMAP FP PDE Evaluation Kit (Molecular Devices P/N R8175) The Molecular Devices' FlexStation 3 is a benchtop multi-mode microplate reader capable of automated fluorescence measurement in multi-well plates. 4. assay development and validation. Ensure yourAccessories for FlexStation® 3 Multimode Plate Reader, Molecular Devices. They were incubated overnight at 37°C, 5% CO 2. このビデオでは、FlexStation 3を用いて、ヒトTRPA1チャネルの薬理学的特性を研究するための詳細なプロトコルを提供します。. The luminescent signal was detected using the FlexStation 3 multi-mode microplate reader (Molecular Devices, San Jose, CA) and data were analyzed by SoftMax Pro 5. With optimized reagents, option to operate the. The tachykinin family is a highly conserved group of multi-functional peptides, sharing a common C-terminal sequence (-Phe-X-Gly-Leu-Met-NH2, where X is a hydrophobic amino acid) . Molecular Devices FlexStation ® 3 Multi-Mode Microplate Reader has integrated fluidics, permitting the realtime measurement of fluorescent and luminescent cell-based assays. SpectraMax® M Series Multi-Mode Microplate . This homogeneous, fluorescent assay is robust, sensitive, specific, and can be run in either a kinetic or an endpoint mode. The plate reader optics are tunable for absorbance. FlexStation 3 combines the performance of Molecular Devices’ SpectraMax M5e with a integrated 8- or 16-channel pipettor into one compact benchtop reader. Latest Citations: For a complete list, please click here. The FlexStation 3 ReaderThe use of the FlexStation® 3 multi-mode microplate reader has been shown to compare favorably to other methods in obtaining quantitative binding affinities between the ß-lactamase and inhibitor protein, TEM-1 and BLIP. The FlexStation 3 Microplate Reader high-efficiency monochromator optics are tunable for absorbance, fluorescence intensity, fluorescence polarisation, and time-resolved fluorescence assays. assay development and validation. application runs on the TRF mode of FlexStation 3 system. Multi-detection platforms often provide assay flexibility, however, throughput is often compromised— especially for applications that require integrated fluid transfer, such as calcium mobilization or other fast applications. features of the FlexStation® 3 Multi-Mode Microplate Reader allows rapid and reliable measurement of EC 50 values for agonists and IC 50 values for antagonists. Here is a Molecular Devices FlexStation 3 Multi-Mode Microplate Reader Unit2 New costs around $125,000 from VWR Predecessor to: Current model Condition: This Molecular Devices FlexStation 3 Multi-Mode Microp. This integrated system provides users with a multi-detection platform capable of increasing the Setup Guide. 1. Backed by the Molecular Devices warranty, these tips are manufactured specifically to work with the FlexStation 3 instrument under strict controls to guarantee optimal performance and quality. Multi-mode microplate readers with luminescence detection. SpectraTestMulti-ModeValidationPlateUserGuide. Condition: Used. Homogeneous Time-Resolved Fluorescence (HTRF) . Thisintegratedsystemprovidesamulti-detectionplatformcapableofincreasingliquid-handlingthroughputandflexibilityfor biochemical-basedandcell-basedassays. The assay plate was allowed to rest at room temperature for 5 min, and luminescence was quantified using the Multi-Mode Microplate Reader (FlexStation 3, Molecular Devices Inc. Kinase Glo detection reagent (10 μl) was then added, followed by incubation for 30 minutes at room temperature in the dark (ADP detection phase). FlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer. To address this concern, Molecular Devices offers the FlexStation® 3 Multi-Mode Microplate Reader. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. FlexStation® 3 Multi-Mode Microplate Reader. But that's just the beginning, because this incredible benchtop system integrates multi-mode microplate optics with. The FlexStation 3 ReaderFor the FlexStation 3 multi-mode microplate reader assay and calcium-imaging assay, HEK293 cells were transiently transfected with a similar mixture but without GFP. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. Published Dec 16, 2020 | Updated Dec 16, 2020. SpectraMax Gemini XPS/EM Microplate Readers. FlexStation 3, a next-generation multi-mode microplate reader, with liquid transfer capabilities provides a robust platform for increased throughput of this important flash luminescence assay. The FlexStation® 3 Multi-Mode Microplate Reader offers added flexibility over dispenser-based systems by directly transferring reagents from a source plate to the read plate, which reduces reagent and consumable consumption. It is ideal for medium- to high-throughput screens in academic settings. ZERO BIAS - scores, article reviews, protocol conditions and more. 6. Products (33) User Reviews (6) Figures (142). The use of the FlexStation® 3 multi-mode microplate reader has been shown to compare favorably to other methods in obtaining quantitative binding affinities between the ß-lactamase and inhibitor protein, TEM-1 and BLIP. . Order Now. . Experienced personnel work in partnership with researchers to develop and/or. Data Data (15) Gasdermin-D activation by SARS-CoV-2 triggers NET and mediate COVID-19 immunopathology Critical Care December 1, 2022 Camila Meirelles S. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research, scientific. Testing we performed:Molecular Devices. 00 USDThe FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. $18,995. Unlike HTR-FRET assays, H188 and YC3. DNA and protein quantitation,. Molecular Devices LMAX II 384 Microplate Reader. A single-mode plate reader is a good starting point. The Molecular Devices' FlexStation 3 is a benchtop multi-mode microplate reader capable of automated fluorescence measurement in multi-well plates. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-Mode Microplate Reader and Gemini Fluorescence Microplate Reader with bottom-read fluorescence detection. . monochromator,multi-modemicroplatereaderandanintegrated8-channelor16-channel pipettorintoonecompactbenchtopreader. 5. This. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield and phase contrast for maximum application reach. Users can choose from 96-well pipetting, 384-well pipetting, or both. Precise optics, fluidic transfer, and assay flexibility on one integrated microplate readerPrice from $9. Questions & Answers. It has an integrated fluid transfer module equipped with a multi-channel pipett. 003% Linearity G08 forMag1Ratio G07 forMag2Ratio G06 forMag3Ratio G05 forMag4Ratio G04 forMag5Ratio G03 forMag6Ratio Mag1Ratio = 0. ** Some compatible filter sets are available for purchase from Life. Learn more. 0 subscHomogeneous Time-Resolved Fluorescence (HTRF) . We would like to show you a description here but the site won’t allow us. Information on cell morphology, localization of signal, cell count, and more is obtained with Cytation 5. . On the. Built on the proven SpectraMax® M5e optical platform, FlexStation 3 expands capacity to perform real time fast kinetic製品名 ピペッター内蔵マルチモードマイクロプレートリーダー FlexStation 3 FLEX3 概算価格：￥15,530,000 96well ピペッターヘッド 0200-6182 概算価格：￥840,000 384well ピペッターヘッド 0200-6183 概算価格：￥1,680,000 マルチチャンネルピペッターを搭載することにより、迅速応答カイネティックにも対応し. . 1 sec. GenePix 4300/4400 Microarray Scanner. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. Multi-detection platforms often provide assay flexibility, however, throughput is often compromised— especially for applications that require integrated fluid transfer, such as calcium mobilization or other fast applications.